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mouse anti myosin heavy chain  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti myosin heavy chain
    Mouse Anti Myosin Heavy Chain, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+myosin+heavy+chain/anti-Myosin+heavy+chain/pmc13089148-163-18-23
    Average 95 stars, based on 18 article reviews
    mouse anti myosin heavy chain - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Immunolabeling:

    Article Title: Immunotolerant Oligomer scaffolds promote regenerative remodeling and improved muscle structure and function after volumetric muscle loss
    Article Snippet: .. Immunolabeling was performed on deparaffinized, antigen-retrieved sections using the following primary antibodies: mouse anti-CD31 (Abcam, Waltham, MA), rabbit anti-neurofilament (Invitrogen, Waltham, MA), rabbit anti-CD68 (Bio-Rad, Hercules, CA), mouse anti-CD11b (Abcam), rabbit anti-myogenin (Abcam), and mouse anti-myosin heavy chain (MHC; DSHB, Iowa City, IA). .. Dual staining employed the ImmPRESS Duet Double Staining HRP/AP Kit (Vector Laboratories, Newark, CA).

    Immunopeptidomics:

    Article Title: Immunotolerant Oligomer scaffolds promote regenerative remodeling and improved muscle structure and function after volumetric muscle loss
    Article Snippet: .. Immunolabeling was performed on deparaffinized, antigen-retrieved sections using the following primary antibodies: mouse anti-CD31 (Abcam, Waltham, MA), rabbit anti-neurofilament (Invitrogen, Waltham, MA), rabbit anti-CD68 (Bio-Rad, Hercules, CA), mouse anti-CD11b (Abcam), rabbit anti-myogenin (Abcam), and mouse anti-myosin heavy chain (MHC; DSHB, Iowa City, IA). .. Dual staining employed the ImmPRESS Duet Double Staining HRP/AP Kit (Vector Laboratories, Newark, CA).

    Incubation:

    Article Title: Hypoxia-induced GRP78 activation disrupts the Fndc5/Irisin axis to accelerate skeletal muscle atrophy
    Article Snippet: The myotubes were subsequently washed three times with PBS containing 0.1% Tween 20 and permeabilized using Triton X-100 (Beyotime; P0096) for 10 min at room temperature. .. After additional rinsing, the cells were incubated with 1% bovine serum albumin (BSA) for 30 min. Primary antibody, mouse anti-myosin heavy chain (MF-20; DSHB), diluted 1:200 in PBST, was applied to the myotubes and incubated overnight at 4°C. .. The cells were washed three times with PBS for 5 mins each before incubation with Alexa FluorTM 488 goat anti-mouse antibody (Invitrogen; R37120 ) diluted in 1% BSA in a light-protected environment at room temperature for 1 h. Nuclei were stained with Hoechst 33258 (Beyotime; C1011) for 10 min.

    Article Title: Vitamin C is essential for proper myogenic differentiation.
    Article Snippet: The prepared samples were loaded at 4.5 μg per lane onto Mini-PROTEAN TGX Gel 4–20% (45680395, Bio-Rad, Hercules, CA, USA), separated by SDS-polyacrylamide gel electrophoresis, and transferred on membranes using a Trans-Blot Turbo Transfer Pack (1704156; Bio-Rad). .. The membrane was Jo urn al Pr e-p oo f 9 blocked with 5% skim milk in tris-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T) for 1 h, and then incubated with mouse anti-myosin heavy chain (MF20, 1:500 dilution, Developmental Studies Hybridoma Bank, Iowa, IA, USA) primary antibody diluted in 5% skim milk in TBS-T overnight at 4°C. .. The membrane was washed with TBS-T and incubated with a horseradish peroxidase-conjugated anti-mouse IgG antibody (Bio-Rad).

    Membrane:

    Article Title: Vitamin C is essential for proper myogenic differentiation.
    Article Snippet: The prepared samples were loaded at 4.5 μg per lane onto Mini-PROTEAN TGX Gel 4–20% (45680395, Bio-Rad, Hercules, CA, USA), separated by SDS-polyacrylamide gel electrophoresis, and transferred on membranes using a Trans-Blot Turbo Transfer Pack (1704156; Bio-Rad). .. The membrane was Jo urn al Pr e-p oo f 9 blocked with 5% skim milk in tris-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T) for 1 h, and then incubated with mouse anti-myosin heavy chain (MF20, 1:500 dilution, Developmental Studies Hybridoma Bank, Iowa, IA, USA) primary antibody diluted in 5% skim milk in TBS-T overnight at 4°C. .. The membrane was washed with TBS-T and incubated with a horseradish peroxidase-conjugated anti-mouse IgG antibody (Bio-Rad).

    Saline:

    Article Title: Vitamin C is essential for proper myogenic differentiation.
    Article Snippet: The prepared samples were loaded at 4.5 μg per lane onto Mini-PROTEAN TGX Gel 4–20% (45680395, Bio-Rad, Hercules, CA, USA), separated by SDS-polyacrylamide gel electrophoresis, and transferred on membranes using a Trans-Blot Turbo Transfer Pack (1704156; Bio-Rad). .. The membrane was Jo urn al Pr e-p oo f 9 blocked with 5% skim milk in tris-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T) for 1 h, and then incubated with mouse anti-myosin heavy chain (MF20, 1:500 dilution, Developmental Studies Hybridoma Bank, Iowa, IA, USA) primary antibody diluted in 5% skim milk in TBS-T overnight at 4°C. .. The membrane was washed with TBS-T and incubated with a horseradish peroxidase-conjugated anti-mouse IgG antibody (Bio-Rad).

    other:

    Article Title: Human neuromuscular organoids mimic cancer-induced muscle cachexia
    Article Snippet: Mouse anti-Myosin Heavy Chain , DSHB , Cat# MF-20; RRID: AB_2147781.



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